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QIAG Epigenetics

 
 
      
Qiagen 1018

QIAGEN – Applications & Insights
Epigenetics

5 Steps from Sample Collection to Analysis

 
QIAG Epigenetics Step 1
Step 1: Sample collection
- For blood collection prior to genomic DNA purification using the PAXgene Blood DNA Kit
 
QIAG Epigenetics Step 2
Step 2: DNA purification
- For purification of up to 12 µg genomic, mitochondrial, or viral DNA from blood and related body fluids
- For isolation of free-circulating DNA and RNA from human plasma or serum
- For spin-column or 96-well purification of total DNA from animal blood and tissues and from cells, yeast, bacteria, or viruses
- For isolation of genomic, mitochondrial, bacterial, parasite or viral DNA
- For purification of genomic DNA from formalin-fixed paraffin-embedded tissues
 
QIAG Epigenetics Step 3
Step 3: Bisulfite conversion
- For ultrafast, highly efficient bisulfite conversion from gDNA, FFPE, blood, cell, or tissue samples
- For ultrafast and efficient bisulfite conversion from various starting materials in a 96-well format
 
QIAG Epigenetics Step 4
Step 4: DNA amplification
- For whole genome amplification of bisulfite converted DNA for PCR analyses
- For PCR amplification of template DNA optimized for Pyrosequencing analysis
 
QIAG Epigenetics Step 5
Step 5: Analysis
- For quantitative analysis of genetic or epigenetic DNA modifications using Pyrosequencing technology
- Predesigned assays for quantification of CpG methylation by Pyrosequencing
- For quantitative, real-time probe-based PCR analysis of methylation status
- For application-focused DNA methylation profiling using MethylScreen technology with laboratory-verified assays
- For application-focused quantitative detection of gene panels in chromatin immunoprecipitation experiments
 
QIAG Epigenetics

Detailed information
 
 

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