NEED HELP?
0800 34 24 66

QIAG Digital PCR

 
 
      
Qiagen 1018

QIAGEN – Applications & Insights
Digital PCR

Digital is the new absolute

 
Finding a needle in a haystack
Researchers performing sensitive applications such as copy number variation and rare target detection often find themselves struggling to identify faint genetic signals against a strong background, especially when it is a single positive lost in a dense pool of negatives. Finding the rare allele or mutant sequence is a typical needle in a haystack problem! By partitioning the sample into a large number of individual reactions, digital PCR makes it surprisingly easy to detect the single positive. It is in such instances where the lower limit of detection excludes standard real-time quantitative PCR as a viable solution.
Digital PCR dPCR inding a needle
   
The power of digital PCR​
Digital PCR is a highly precise approach to sensitive and reproducible nucleic acid detection and quantification. Measurements are performed by dividing the sample into partitions, such that there is either zero or one target molecule present in any individual reaction. Each partition is analyzed after end-point PCR cycling for the presence or absence of a fluorescent signal, and the absolute number of molecules present in the sample is calculated. It does not rely on a standard curve for sample target quantification. Eliminating the reliance on standard curve reduces error and improves precision. 
Digital PCR dPCR header sm
 

foot-logo

Bio-Strategy’s team of highly qualified professionals share a passion for science. In partnership with world leading suppliers we provide quality technology solutions for research, biotechnology, clinical diagnostics and industrial applications.

Read More 
     
Contact Us
New Zealand: 0800 34 24 66